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KMID : 0359320130530010037
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Korean Journal of Veterinary Research
2013 Volume.53 No. 1 p.37 ~ p.42
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Characterization of multipotent mesenchymal stem cells isolated from adipose tissue and bone marrow in pigs
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Lee Ah-Young
Choe Gyeong-Im
Nah Jin-Ju
So Byung-Jae
Lee Kyung-Woo
Chang Ki-Yoon
Song Jae-Young
Cha Sang-Ho
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Abstract
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Mesenchymal stem cells (MSCs) have ability to differentiate into multi-lineage cells, which confer a great promise for regenerative medicine to the cells. The aim of this study was to establish a method for isolation and characterization of adipose tissue-derived MSC (pAD-MSC) and bone marrow-derived MSC (pBM-MSC) in pigs. Isolated cells from all tissues were positive for CD29, CD44, CD90 and CD105, but negative for hematopoietic stem cell associated markers, CD45. In addition, the cells expressed the transcription factors, such as Oct4, Sox2, and Nanog by RT-PCR. pAD-MSC and pBM-MSC at early passage successfully differentiated into chondrocytes, osteocytes and adipocytes. Collectively, pig AD-MSC and BM-MSC with multipotency were optimized in our study.
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KEYWORD
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mesenchymal stem cells, isolation, pig, adipose tissue, bone marrow
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